InterFase Plus® provides the same unique enzyme formulation as InterFase® with the addition of ethylenediaminetetraacetic acid (EDTA). The presence of calcium, iron, and magnesium is essential for biofilm creation and serves to cross-link the anionic regions for polymers. InterFase Plus® includes EDTA which binds the metals needed for biofilm formation. Biofilm consists of microorganisms encased within a self-produced matrix of exopolysaccharides and exoproteins that strongly adheres to interfaces and resists dislodgement. Microorganisms residing within biofilms are highly resistant to antimicrobials including antibiotics and bacteriocins produced by probiotics. InterFase Plus® is a unique enzyme formulation with EDTA that is especially designed to disrupt the biofilm matrix that embeds gastrointestinal organisms. Enzymes in InterFase Plus® are selected for their ability to lyse the extracellular polymers commonly found in biofilm as well as degrade bacterial and yeast cell wall structures. The enzyme blend in InterFase Plus® was developed using in vitro testing of antibiofilm activity and was found to have significant antibiofilm activity resulting in meaningful degradation of undesirable biofilm communities. InterFase Plus® is intended for use in conjunction with efforts to support normal gastrointestinal function and microbiota. As an adjunct, it is best combined with use of a high-potency, broad-spectrum, multispecies probiotic formulation and a prebiotic to encourage the formation of healthy intestinal microbial biofilm communities. The capsules should be taken away from meals to maximize the enzyme effects.